Protocols
The protocols listed below are VMSR-suggested protocols that work well in our hands. Individual results may differ and we encourage optimization for individual lab conditions.
- General Protocols:
- General Slide Handling Protocol
- PCR Amplification of NIA Clonesets
- RNA Isolation Protocols:
- RNA Isolation Protocol for Cultured Cell Samples
- RNA Isolation Protocol for Tissue Samples
- cDNA Labeling Protocols:
- Standard Protocol 30-50µg total RNA
- Indirect Protocol (coupling) As low as 10µg total RNA
