Submitting Samples to the VMSR
- Isolate your sample according to the protocols on the sample prep page.
- Load at least the amount specified for your platform in the table below into a 1.5ml tube. This table lists sample requirements, not guidelines. Samples outside the range specified here will be subject to an extra sample handling fee
Platform Concentration Range Total Volume Gene Expression (total RNA) Affymetrix 3' 250-2000 ng/µl 10 µl Affymetrix Gene 50-100 ng/µl 5 µl Affymetrix Exon 625-1000 ng/µl 5 µl Agilent 100 ng/µl 7 µl miRNA Expression 500-1000 ng/µl 5 µl Nugen Amplification 5-20 ng/µl 10 µl Genotyping (genomic DNA) DNA Mapping 50 ng/µl 20 µl Targeted Genotyping (<= 5K) 150 ng/µl 15 µl Targeted Genotyping (> 5K) 150 ng/µl 30 µl Resequencing 5 ng/µl 25 µl Real-time PCR (total RNA) TaqMan Assays (per assay) 50+ ng/µl 5 µl Low-Density Arrays (per port) 50+ ng/µl 5 µl Nugen Amplification 5-20 ng/µl 10 µl RNA QC/QA Projects Please submit at least 5 µl If samples are precious, contact us before submitting - Log in to the VMSR Project database (in the green bar on the right)
- Select the appropriate project from your My Projects list. For more information about VMSR Projects, see the User's Manual
- Click the "Submit New Samples" button, and complete the forms there. For Real-time PCR projects, follow the instructions on your project page
- After you place your order, you'll receive an email with a completed Submission Sheet (in PDF form). Print out this sheet.
- Label your tubes with the sample number from the sheet on the top, and the date on the side. Please do not use tape.
- Bring your samples, along with the printed out sheet, to the VMSR during standard business hours. If you're not at Vanderbilt, follow the instructions on the Shipping Samples page
- Your samples will be assessed for quantity and quality within three business days (unless they are DNA)
- When you receive your QA results, place a Hybridization order for the samples you wish to proceed with
- Within two weeks, you'll receive your expression data (genotyping results take longer)
